Evaluation of recombinant leptospiral surface antigen (Lsa27) lipoprotein for serodiagnosis of human leptospirosis by latex agglutination test
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چکیده
منابع مشابه
Evaluation of recombinant leptospiral antigen LipL41 in enzyme- linked immunosorbent assay and latex agglutination test for serodiagnosis of canine leptospirosis
Leptospirosis is an important global veterinary health problem caused by spirochetes belonging to the genus Leptospira. Leptospires are ubiquitous in nature, and therefore able to adapt to both the ambient environment and the renal tubules of chronically infected reservoir hosts. Microscopic agglutination test (MAT) remains a specialized test, which is not generally performed in the routine dia...
متن کاملSimple latex agglutination assay for rapid serodiagnosis of human leptospirosis.
A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agglutination test was evaluated with groups of serum samples from patients with ...
متن کاملDevelopment and evaluation of a latex agglutination test for the serodiagnosis of paracoccidioidomycosis.
Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. It is caused by the dimorphic fungus Paracoccidioides brasiliensis. The immunodiffusion (ID) test is one of the most widely used techniques for PCM serologic diagnosis due to the simplicity and low costs of its execution. However, it requires trained and qualified people to execute it. The purpose of this stud...
متن کاملEvaluation of smooth lipopolysaccharide based latex agglutination test in serodiagnosis of brucellosis
In this study, sLPS antigen coated latex bead was prepared and their evaluation was done along with RBPT, commonly employed for the serodiagnosis of brucellosis. sLPS was extracted from Brucella abortus S99 and coated on polystyrene latex beads in a phosphate buffer saline coating buffer. A total of 200 samples were collected from unorganized dairy farm and tested with RBPT and sLPS based latex...
متن کاملEvaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
There is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96%...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2021
ISSN: 0255-0857
DOI: 10.1016/j.ijmmb.2021.01.006